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Edexcel International GCSE Biology (4BI1) · Ecology & the Environment
Section 4

Ecology & the Environment

Everything alive is connected — who eats whom, how energy runs out along the way, and how carbon and nitrogen get recycled forever.

In this mini-lesson you'll master the whole of Edexcel 4BI1 Section 4: populations & sampling, food webs, ecological pyramids, energy flow, the carbon cycle, the nitrogen cycle, and human impacts — pollution, eutrophication and deforestation.

Watch for the purple tag: Biology only (Paper 2) marks content examined only in International GCSE Biology, not in Double Award Science (the nitrogen cycle stages and the effects of deforestation). 4BI1 is untiered.

Make a guess: how could you estimate the daisies fairly?

  1. Mission 1How do you count daisies without counting?
  2. Mission 2Why do food chains stop after a few links?
  3. Mission 3Where does the carbon in a rotting leaf go?
  4. Mission 4How can fertiliser kill the fish in a lake?

Tap Start, answer each check to unlock the next screen, and collect ⭐ as you go.

The organism in the environment

Population, community, habitat, ecosystem

Ecology uses a ladder of precise words. Get them exact:

  • Habitat — the place where an organism lives (e.g. a rock pool).
  • Population — all the organisms of one species in a habitat at one time.
  • Community — all the populations of all the species living together.
  • Ecosystem — the community plus the non-living (abiotic) physical environment, interacting together.
ECOSYSTEM (community + physical environment) COMMUNITY (all populations) POPULATION one species POPULATION another species ☀️ 💧 🌡️ abiotic factors: light, water, temperature
The levels nest inside each other: populations → community → ecosystem.
Quick check

Which word fits?

?All the perch fish, all the reeds, all the frogs and all the water beetles living in one pond form a…
Sampling

Quadrats — estimating population size

You can't count every daisy on a field, so you sample. A quadrat is a square frame (often 0.5 m × 0.5 m) placed at random positions.

  • Count the organisms inside several quadrats placed at random coordinates.
  • Find the mean number per quadrat.
  • Scale up: mean per quadrat × (total area ÷ quadrat area) = estimated population size.

Why random? Random placement avoids bias and makes the sample representative. More quadrats → more reliable estimate.

Worked example

10 quadrats (each 0.25 m²) hold 40 daisies in total, in a field of 500 m².

Mean = 40 ÷ 10 = 4 per quadrat. Field holds 500 ÷ 0.25 = 2000 quadrat-areas.

Estimated population = 4 × 2000 = 8000 daisies.

Calculate

Your turn — estimate the population

1A student places 8 quadrats and counts 24 dandelions in total. The whole lawn is 20 times the area of one quadrat. Estimate the number of dandelions on the lawn.
dandelions
Hint: mean per quadrat = 24 ÷ 8, then × 20.
Sampling

Belt transects — measuring distribution

A transect is a line across a habitat. To study how species change with distance (e.g. up a beach), you lay a tape and place quadrats at regular intervals along it — a belt transect.

sea land ↑ tape measure (transect line) 0 m 5 m 10 m 15 m
Quadrats at fixed intervals along the tape record how species abundance changes with distance.

Use a transect when a physical factor changes across the habitat (light, water, salinity). Use random quadrats for an overall estimate of one uniform area.

Quick check

Right tool for the job

?You want to see how seaweed cover changes as you go from the low-tide mark up to the top of a rocky shore. Best method?
Mission 1 check

How do you count daisies without counting?

?A student places ten 0.25 m² quadrats at random on a 400 m² field. She counts 30 daisies in total. Estimate the number of daisies on the field.
Mission 2 of 4

Why do food chains stop after a few links?

Make a guess: why do food chains stop after a few links?

Feeding relationships

Producers, consumers & decomposers

  • Producers — plants (and algae) that photosynthesise to make their own food. They start every food chain.
  • Consumers — animals that eat other organisms. Primary eat producers, secondary eat primary, tertiary eat secondary.
  • Decomposers — bacteria and fungi that break down dead material and waste, recycling nutrients back into the soil.

The stages (producer, primary consumer, secondary consumer…) are called trophic levels.

grass → grasshopper → lizard → hawkproducer → primary → secondary → tertiary consumer. The arrow → means "is eaten by".

Misconception buster: the arrow points in the direction that energy and biomass flow — from the food to the feeder. It does not point at what the animal eats.

Feeding relationships

Food chains link into a food web

Most organisms eat more than one thing, so several chains cross to make a food web. Follow the arrows: each one shows energy passing from prey to predator.

🌱 grass 🌾 seeds 🍃 leaves 🐇 rabbit 🐭 mouse 🐛 caterpillar 🦊 fox 🦉 owl
Every arrow points from the eaten to the eater — the direction energy flows.
Quick check

Read the web

?In the food web above, an arrow runs from the mouse to the owl. What does this arrow tell you?
Mini-game

Match organism ↔ trophic level

Tap an organism on the left, then its correct role on the right.

Organism
Trophic level
Feeding relationships

Ecological pyramids

  • Pyramid of numbers — the number of organisms at each level. Can be an odd shape (one oak tree feeds many aphids, so the base is narrow).
  • Pyramid of biomass — the total mass of living material at each level. Almost always a true pyramid.
  • Pyramid of energy transfer — the energy passed to each level per year. Always a true pyramid.
tertiaryconsumers40 g/m² secondary(foxes)120 g/m² primary(rabbits)400 g/m² producers(grass)1200 g/m² bar width drawn to scale for biomass
Biomass falls steeply at each level (here to about a third) — that's why it tapers.

Key idea: the width of each bar is drawn to scale for the mass (or energy) at that level — not the number of organisms.

Calculate

Your turn — biomass transfer

1Producers in a meadow hold 12 000 g/m² of biomass. If about 10% passes to the primary consumers, what biomass do they hold?
g/m²
Hint: 10% of 12 000 = 12 000 ÷ 10.
Energy flow

Why only ~10% gets through

Only about 10% of the energy at one trophic level ends up stored in the next. The other ~90% is lost because:

  • Much is released as heat during respiration (movement, warmth).
  • Not all of the prey is eaten (bones, roots) or is indigestible — lost in faeces.
  • Some is lost in excretory waste (urine).
producer1000 kJ primary100 kJ secondary10 kJ 10%10% ~90% lost at each step: heat (respiration), faeces, urine
Because ~90% is lost per step, food chains are usually short (rarely more than 4–5 links).
Quick check

Why so short?

?Food chains rarely have more than four or five links. What is the best explanation?
Mission 2 check

Why do food chains stop after a few links?

?On an island, grass is eaten by grasshoppers, grasshoppers by lizards, and lizards by snakes. No animal there feeds mainly on the snakes. What best explains this?
Mission 3 of 4

Where does the carbon in a rotting leaf go?

Make a guess: where does the carbon in a rotting leaf go?

Cycles within ecosystems

The carbon cycle

Carbon moves between the air and living things. Learn the four core processes:

  • Photosynthesis removes CO₂ from the air, fixing carbon into glucose.
  • Respiration (by plants, animals and decomposers) returns CO₂ to the air.
  • Combustion — burning fossil fuels and wood returns CO₂.
  • Decomposition — microbes respire dead matter, releasing CO₂; incomplete decay over millions of years forms fossil fuels.
CO₂ in the atmosphere 🌳 plantsproducers 🐄 animalsconsumers decomposers(microbes) ⛽ fossil fuels photo-synthesis respiration respiration respiration feeding death death combustion
Photosynthesis takes carbon in; respiration and combustion give it back.
Quick check

Carbon out of the air

?Which single process removes carbon dioxide from the atmosphere?
Mission 3 check

Where does the carbon in a rotting leaf go?

?A fallen leaf rots on the forest floor. Months later, some of its carbon is part of a new leaf on a nearby tree. Which route did the carbon most likely take?
Biology only (Paper 2)

The nitrogen cycle

Plants need nitrogen (for proteins) but can't use nitrogen gas (N₂) — they absorb nitrates through their roots. Four groups of bacteria move nitrogen around:

  • Nitrogen-fixing bacteria — turn N₂ from the air into ammonia/ammonium (some live in root nodules of legumes).
  • Decomposer bacteria — break down dead matter and urea into ammonium.
  • Nitrifying bacteria — convert ammonium → nitrites → nitrates (needs oxygen).
  • Denitrifying bacteria — convert nitrates back into N₂ gas (in waterlogged, low-oxygen soil).
N₂ gas in the atmosphere ammonium(NH₄⁺) nitrates(NO₃⁻) 🌱 plantproteins dead matter& urea N-fixingbacteria denitrifyingbacteria nitrifying bacteria (via nitrites) absorbed by roots death decomposers
Nitrogen-fixing: N₂ → ammonium; nitrifying: ammonium → nitrates; plants absorb nitrates; denitrifying: nitrates → N₂.
Paper 2 check

Name that bacterium

?Which bacteria convert ammonium ions in the soil into nitrates that plants can absorb?
Mini-game

Which cycle is this process in?

Tap whether each process belongs to the carbon cycle or the nitrogen cycle.

Mission 4 of 4

How can fertiliser kill the fish in a lake?

Make a guess: how can fertiliser end up killing the fish?

Human influences — air pollution

Greenhouse gases & global warming

Human activities raise the levels of greenhouse gases: burning fossil fuels and deforestation add carbon dioxide (CO₂); cattle, rice paddies and landfill add methane (CH₄).

  • They trap heat radiated from the Earth (the greenhouse effect); more of them trap more heat (an enhanced greenhouse effect), raising average temperatures — global warming.
  • Edexcel's list of greenhouse gases: water vapour, CO₂, nitrous oxide, methane and CFCs.
  • Consequences: melting ice, rising sea levels, changing climate and habitats.

Don't confuse: the greenhouse effect (heat trapping → warming) is different from acid rain (sulfur dioxide) and different again from the ozone story — Edexcel 4BI1 wants greenhouse gases → enhanced greenhouse effect → warming.

Human influences — air pollution

Sulfur dioxide & acid rain

Burning fossil fuels (especially coal) releases sulfur dioxide (SO₂). It dissolves in rain water to form acids, giving acid rain.

  • Acid rain damages leaves and kills trees.
  • It makes lakes and rivers acidic, killing fish and other aquatic life.
  • It erodes limestone buildings and statues.
SO₂ + rain water → acid rainSulfur dioxide (and nitrogen oxides) dissolve in rain, lowering its pH.

Also on the spec — carbon monoxide (CO): from incomplete combustion of fuels. It binds to haemoglobin in red blood cells, so the blood carries less oxygen; high levels are fatal.

Quick check

Pollutant ↔ problem

?Which gas, released mainly by burning coal, is the chief cause of acid rain?
Human influences — water pollution

Eutrophication

When excess nitrate/phosphate fertiliser (or untreated sewage) washes into a river or lake, it triggers a chain of events:

  1. Nutrients cause an algal bloom — algae grow rapidly over the surface.
  2. Algae block light, so plants below die.
  3. Decomposer bacteria feed on the dead plants and multiply.
  4. These bacteria respire aerobically, using up the dissolved oxygen.
  5. Fish and other animals die from lack of oxygen.
1. fertiliser (nitrates) washes in 2. algal bloom blocks the light 3. plants below die; bacteria multiply 4. bacteria respire — O₂ used up 5. fish suffocate 🐟
The killer step: decomposer bacteria use up the water's oxygen.

Misconception buster: the fertiliser doesn't poison the fish directly — the fish suffocate because bacteria remove the oxygen from the water.

Explain it

Your turn — why the fish die

?In eutrophication, what is the immediate cause of the fish dying?
Mission 4 check

How can fertiliser kill the fish in a lake?

?Fertiliser washes into a lake and algae grow fast. A scientist finds that the dissolved oxygen falls sharply only after the algae start to die. Why does it fall then?
Human influences Biology only (Paper 2)

Deforestation

Cutting down forests (often by slash-and-burn) has several consequences Edexcel expects you to list:

  • Less photosynthesis → less CO₂ removed from the air (and less O₂ released) → more global warming.
  • Burning trees releases stored carbon as CO₂ (combustion).
  • Loss of habitat and reduced biodiversity (species may go extinct).
  • Soil erosion, leaching of minerals from the soil, and flooding — tree roots no longer bind the soil.
  • Disruption of the water cycle — less evapotranspiration, drier local climate.
Calculate

Your turn — habitat lost

1A rainforest covered 5000 hectares. Deforestation removed 35% of it. How many hectares of forest are now left?
hectares
Hint: 100% − 35% = 65% remains, so 0.65 × 5000.
Human influences

Conservation — protecting biodiversity

Humans can reduce their impact and protect species and habitats:

  • Protecting habitats — nature reserves and national parks.
  • Replanting forests and managing woodland sustainably.
  • Captive breeding and seed/gene banks to save endangered species.
  • Reducing pollution — treating sewage, controlling fertiliser use, cutting emissions.
  • Recycling nutrients and materials to reduce waste and habitat destruction.

Big picture: conservation keeps ecosystems working — food webs, the carbon cycle and the nitrogen cycle all depend on biodiversity being maintained.

Recap

You've covered the key ideas in Section 4 ✅

Organisms & environment: population < community < ecosystem; quadrats estimate size, belt transects show distribution.

Feeding: producers → consumers → decomposers; arrows = energy flow; food webs; pyramids of number, biomass & energy.

Energy flow: ~10% passes on, ~90% lost as heat/faeces/waste → short chains.

Cycles: carbon (photosynthesis, respiration, combustion, decomposition); nitrogen Paper 2 (fixing, decomposer, nitrifying, denitrifying bacteria).

Human impact: CO₂/CH₄ → global warming; SO₂ → acid rain; eutrophication (O₂ depletion kills fish); deforestation; conservation.

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