A-Level Biology Revision

Energy, Respiration & Photosynthesis

Photosynthesis and respiration — the biochemistry of how cells capture, store and release energy.

Energy, Respiration & Photosynthesis is a core part of A-Level Biology. Revise the key concepts and common mistakes below, then lock them in with the free games.

Key concepts

GlycolysisSplits 1 glucose into 2 pyruvate in the cytoplasm — net 2 ATP, 2 NADH
PhosphorylationAdding a phosphate (often from ATP) to make a molecule more reactive
Pyruvate3-carbon product of glycolysis
Link reactionPyruvate → acetyl CoA + CO₂ + NADH in mitochondrial matrix
Coenzyme ACarrier that hands the 2-carbon acetyl group to the Krebs cycle
Substrate-level phosphorylationDirect ATP synthesis from a phosphorylated substrate
Anaerobic respirationRespiration without oxygen — much lower ATP yield
Lactate fermentationPyruvate + NADH → lactate + NAD (mammalian muscle)
Ethanol fermentationPyruvate → CO2 + ethanal → ethanol + NAD (yeast)
Oxygen debtLactate later transported to liver, oxidised back to pyruvate
Respiratory quotientRQ = CO2 produced ÷ O2 consumed — varies with substrate
Lipids as fuelMore ATP per gram than carbs, but slower to release
Krebs cycleMatrix cycle starting with citrate (6C) — produces NADH, FADH2, ATP, CO2
Electron transport chainSeries of carriers on the inner mitochondrial membrane

Common mistakes to avoid

Questions where students often pick the tempting wrong answer — make sure you know the right one:

What is cellular respiration?✗ Respiration means breathing — inhaling oxygen and exhaling carbon dioxide.   ✓ A series of chemical reactions in cells that break down glucose to release energy as ATP.
Why is ATP described as an 'energy currency' rather than energy storage?✗ Cells store large amounts of ATP for emergency use.   ✓ ATP is constantly made and broken down — it transfers energy between reactions rather than storing large amounts for later.
Why are food chains usually limited to about 4 or 5 trophic levels?✗ There aren't enough types of animals to fill more trophic levels.   ✓ Only ~10 percent of energy is transferred between trophic levels; eventually too little energy remains to support another level.
What is produced in anaerobic respiration in human muscle cells?✗ Anaerobic respiration produces ethanol and CO2 in humans.   ✓ Lactic acid (lactate) and a small amount of ATP — no CO2 or water in this pathway.
Why is pyruvate reduced to lactate in muscles during anaerobic respiration?✗ To produce more ATP   ✓ To regenerate NAD so glycolysis continues

Practise Energy, Respiration & Photosynthesis — free games

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