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Cambridge IGCSE Biology (0610) · Biological molecules
Mini-Lesson

Biological molecules

This mini-lesson covers Topic 4 — Biological molecules: the elements and building blocks of carbohydrates, proteins and fats, the food tests (iodine, Benedict's, biuret, ethanol emulsion and DCPIP) and the structure of DNA.

molecules & building blocks food tests DNA the chemicals that make up living things

Work through each screen, answer the questions as you go and collect ⭐ stars. Watch for the Supplement flag. Press Start when you're ready.

Molecules · elements & building blocks

Carbohydrates, proteins & fats

Large biological molecules are built from smaller ones:

  • Carbohydrates — contain carbon, hydrogen and oxygen (C, H, O). Starch, glycogen and cellulose are all built from glucose.
  • Proteins — contain carbon, hydrogen, oxygen and nitrogen (C, H, O, N; some also contain sulfur). Proteins are built from amino acids.
  • Fats and oils (lipids) — contain carbon, hydrogen and oxygen (C, H, O). They are built from fatty acids and glycerol.
glucose → starch · amino acids → protein · fatty acids + glycerol → fatlarge molecules are assembled from these smaller sub-units

Exam tip: the extra element in proteins is nitrogen — that's the easy way to tell proteins apart from carbohydrates and fats, which are all just C, H and O.

Quick check

The extra element

?Which element is always present in proteins but not in carbohydrates or fats?
Match it

Match the molecule to its building block

Tap a large molecule on the left, then the sub-unit it is built from on the right.

Large molecule
Built from
Food tests

The food tests

Each nutrient has a chemical test with a colour change from negative to positive:

  • Starch — add iodine solution: orange-brown → blue-black if starch is present.
  • Reducing sugars (e.g. glucose) — add Benedict's solution and heat: blue → brick-red/orange if present.
  • Protein — add biuret solution: blue → purple/violet if protein is present.
  • Fats & oils — the ethanol emulsion test: a milky-white cloudy emulsion forms if fat is present.
  • Vitamin C — add to blue DCPIP: it decolourises (goes colourless) if vitamin C is present.
starchblue-black sugarbrick-red proteinpurple fatmilky vit Ccolourless
Positive results: starch blue-black · sugar brick-red · protein purple · fat milky emulsion · vitamin C decolourises DCPIP.

Watch out: only Benedict's needs heating. Benedict's detects reducing sugars — not all sugars — and starch gives a negative Benedict's result.

Quick check

Reading a food test

?A student adds iodine solution to a food sample and it turns blue-black. What does this show?
Sort it

Which nutrient does each test find?

Tap a test result, then tap the nutrient it detects.

🍞 Starch

🍬 Reducing sugar

🥚 Protein

Quick check

The odd one out

?Which food test requires the sample to be heated (e.g. in a water bath) to get a result?
Supplement · DNA structure

The structure of DNA

DNA is the molecule that carries genetic information. Its structure:

  • Two strands coiled together to form a double helix.
  • Each strand contains chemicals called bases.
  • Bonds between pairs of bases hold the two strands together.
  • The bases always pair up in the same way: A with T, and C with G (complementary base pairing).
AT CG TA GC • two strands = double helix • bases pair: A–T and C–G • bonds between base pairs
Complementary base pairing: A always pairs with T, and C always pairs with G.
Supplement · Calculate

Your turn — base pairing

1One strand of a short piece of DNA has the base sequence A T C G A T. Because A pairs with T and C pairs with G, how many of the bases on the opposite strand are T?
bases
Hint: each A on this strand pairs with a T opposite. Count the A's: A T C G A T.
Supplement · Quick check

Which base pairs with C?

?In DNA, which base always pairs with cytosine (C)?
Recap

The big ideas to know

Carbohydrates: C, H, O · from glucose (starch, glycogen, cellulose)

Proteins: C, H, O, N · from amino acids

Fats/oils: C, H, O · from fatty acids + glycerol

Food tests: iodine→starch (blue-black) · Benedict's + heat→reducing sugar (brick-red) · biuret→protein (purple) · ethanol emulsion→fat (milky) · DCPIP→vitamin C (decolourises)

DNA (Supp): double helix, two strands, bases pair A–T and C–G

You've covered all of Cambridge IGCSE Topic 4. Press Finish to see your score.

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